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J Virol. 1972 June; 9(6): 973-980
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Synthesis In Vitro of Type 5 Adenovirus Capsid Proteins ¹

^a Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104

ABSTRACT

Reaction mixtures containing cytoplasmic extracts and ribosomal fractions prepared from KB cells infected with type 5 adenovirus were able to carry out incorporation of amino acids into protein. The in vitro product included proteins which reacted specifically with antisera to adenovirus capsid proteins; in control experiments with extracts from uninfected cells, no reactions with the antisera were found. The viral proteins were synthesized in vitro on small polyribosomes, were released from them, and significant numbers of the free polypeptides were assembled in vitro into multimeric adenovirus capsid structures.

¹ Presented in part at the 71st Annual Meeting of the American Society for Microbiology, 2 to 7 May 1971, Minneapolis, Minn.