This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Robb, J. A. Articles by Martin, R. G. Search for Related Content PubMed PubMed Citation Articles by Robb, J. A. Articles by Martin, R. G.

 Previous Article  |  Next Article 

J Virol. 1972 June; 9(6): 956-968
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Genetic Analysis of Simian Virus 40 III. Characterization of a Temperature-Sensitive Mutant Blocked at an Early Stage of Productive Infection in Monkey Cells ¹

^a Department of Pathology, University of California, San Diego, School of Medicine, La Jolla, California 92037
^b Laboratory of Molecular Biology, National Institute of Arthritis and Metabolic Diseases, Bethesda, Maryland 20014

ABSTRACT

A temperature-sensitive mutant of simian virus 40 (SV40), ts^*101, has been characterized during productive infection in monkey kidney cells. The mutant virion can adsorb to and penetrate the cell normally at the restrictive temperature, but cannot induce the synthesis of cellular deoxyribonucleic acid (DNA) nor initiate the synthesis of SV40-specific tumor, virion, or U antigens or viral DNA. First-cycle infection with purified ts^*101 DNA is normal at the restrictive temperature, but the resulting progeny virions are still temperature-sensitive. The mutant neither complements nor inhibits other temperature-sensitive SV40 mutants or wild-type virions. The affected protein in the ts^*101 mutant may be a regulatory structural protein, possibly a core protein, that is interacting with the viral DNA.

¹ An abstract of some of this material has been presented previously (J. A. Robb. 1970. J. Cell Biol. 47: part 2, p. 172a).

This article has been cited by other articles:

• Nathans, D (1979). Restriction endonucleases, simian virus 40, and the new genetics. Science 206: 903-909