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J Virol. 1972 June; 9(6): 891-902
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Virus-Specific Ribonucleic Acid in Cells Producing Rous Sarcoma Virus: Detection and Characterization

Jo-Ann Leong, Axel-Claude Garapin, Nola Jackson, Lois Fanshier, Warren Levinson and J. Michael Bishop

1 Department of Microbiology, University of California, San Francisco, California 94122

ABSTRACT

Cells producing Rous sarcoma virus contain virus-specific ribonucleic acid (RNA) which can be identified by hybridization to single-stranded deoxyribonucleic acid (DNA) synthesized with RNA-directed DNA polymerase. Hybridization was detected by either fractionation on hydroxyapatite or hydrolysis with single strand-specific nucleases. Similar results were obtained with both procedures. The hybrids formed between enzymatically synthesized DNA and viral RNA have a high order of thermal stability, with only minor evidence of mismatched nucleotide sequences. Virus-specific RNA is present in both nuclei and cytoplasm of infected cells. This RNA is remarkably heterogeneous in size, including molecules which are probably restricted to the nucleus and which sediment in their native state more rapidly than the viral genome. The nature of the RNA found in cytoplasmic fractions varies from preparation to preparation, but heterogeneous RNA (ca. 4-50S), smaller than the viral genome, is always present in substantial amounts.

J Virol. 1972 June; 9(6): 891-902
Copyright © 1972 American Society for Microbiology. All Rights Reserved.



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