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J Virol. 1972 June; 9(6): 1033-1046
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Organization of Gene Function in Bacillus subtilis Bacteriophage SP82G ¹

^a Department of Biochemistry, University of New Hampshire, Durham, New Hampshire 03824
^b Department of Biophysics and Microbiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15200

ABSTRACT

A generalized assessment of the functions of 26 genes of SP82G, a bacteriophage of Bacillus subtilis, has been made. The production of phage-specific deoxyribonucleic acid (DNA), DNA-filled phage heads, completed phage particles, phage-specific antigen, and developmental aberrations has been examined in lysates of temperature-sensitive mutants grown under selective conditions. The genes show a tendency to occur on the genome in three groups of related function: genes involved with DNA synthesis, with tail synthesis, and with head synthesis.

² Present address: Department of Biochemistry, The University of New Hampshire, Durham, N.H. 03824.

³ Present address: Presbyterian-University Hospital, Pittsburgh, Pa. 15213.

¹ Publication no. 563 of the Agricultural Experiment Station of the University of New Hampshire, Durham, N.H.