JVI Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Souza, K. A.
Right arrow Articles by Haight, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Souza, K. A.
Right arrow Articles by Haight, R. D.

 Previous Article  |  Next Article 

J Virol. 1972 May; 9(5): 851-856
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Isolation of a Polyvalent Bacteriophage for Escherichia coli, Klebsiella pneumoniae, and Aerobacter aerogenes1

K. A. Souza, H. S. Ginoza and R. D. Haight

Biological Adaptation Branch, Ames Research Center, National Aeronatics and Space Administration, Moffett Field, California 94035
Department of Biology, San Jose State College, San Jose, California 95114

ABSTRACT

A lytic bacteriophage isolated from sewage was found to attack strains of Aerobacter aerogenes, Escherichia coli, and Klebsiella pneumoniae, but not members of the genera Salmonella, Proteus, and Serratia. The phage, designated {varphi}mp, contained deoxyribonucleic acid with a 50% guanine plus cytosine ratio and a molecular weight of 23.1 x 106 daltons. Single-step growth experiments of {varphi}mp plated at 37 C on A. aerogenes A2 gave a mean latent period of 20 min, an average burst size of 103 plaque-forming units/infected cell, and an average adsorption rate constant of 3 x 10–10 ml/min. Electron microscopy of {varphi}mp revealed a phage with a flexible tail (165 nm long and 6 nm wide). The phage head had a hexagonal outline (62 nm in diameter).

FOOTNOTES

1 Submitted by K.A.S. in partial fulfillment of the requirements for the degree Master of Arts at San Jose State College, San Jose, Calif. A preliminary report of this work was presented at the 71st Annual Meeting of the American Society for Microbiology, Minneapolis, Minn., 2-7 May 1971.

J Virol. 1972 May; 9(5): 851-856
Copyright © 1972 American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Mol. Cell. Biol. Microbiol. Mol. Biol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1972 by the American Society for Microbiology. All rights reserved.