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J Virol. 1972 May; 9(5): 842-850
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Solubilization of Envelopes of HVJ (Sendai virus) with Alkali-Emasol Treatment and Reassembly of Envelope Particles with Removal of the Detergent

^a Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Suita City, Osaka, Japan

ABSTRACT

The envelopes of HVJ (Sendai virus) virions were solubilized with alkali-Emasol treatment. The solubilized envelope subunit(s) associated with hemagglutination-inhibiting antibody blocking, neuraminidase, and low hemagglutinating (HA) activities had a sedimentation coefficient of 8.8S. Envelope fragment-like structures were assembled from the solubilized subunits after Emasol was removed by gel filtration. These reassembled envelope particles with HA activity had cell-fusion activity as well as hemolytic activity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the reassembled particles revealed that they mainly consisted of two kinds of polypeptides.

¹ Present address: Department of Bacteriology, Tohoku University School of Medicine, Sendai, Japan.