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J Virol. 1972 May; 9(5): 813-822
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Effect of Sendai Virus Infection on Lipid Metabolism in Chick Embryo Fibroblasts

^a Departments of Bacteriology and Biochemistry, Trinity College, Dublin, Ireland

ABSTRACT

Lipid metabolism in the "abortive" system, Sendai virus-infected primary chick embryo fibroblasts, was examined by using ³²P-orthophosphate, ¹⁴C-glucose, and ¹⁴C-glycerol as precursors. Incorporation of radioactivity from ³²P-orthophosphate and ¹⁴C-glucose into lipid was increased in infected cells. Synthesis of all individual phospholipids was about equally stimulated. There was also evidence for increased lipid synthesis in more productively infected monkey kidney cells. Incorporation of ¹⁴C-glycerol when at a high level in the medium was also increased. However, when this precursor was supplied in minute quantities of high specific activity, incorporation was inhibited. Even though incorporation of radioactivity from ¹⁴C-glucose was stimulated during long labeling periods, the uptake of this precursor during short pulses was inhibited in infected cells. The phenomenon of increased labeling of triglyceride in infected chick cells under certain conditions is discussed, in conjunction with the other effects, in terms of related changes in other virus-infected systems.

¹ Present address: Department of Biochemistry, University College, Dublin 4, Ireland.