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J Virol. 1972 May; 9(5): 776-784
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Nucleic Acid Synthesis in Bacteriophage SPO2c1-Infected Bacillus subtilis

P. E. Kolenbrander1, H. Ernest Hemphill and H. R. Whiteley

Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington 98195
Department of Biology, Syracuse University Syracuse, New York 13210

ABSTRACT

The synthesis of host macromolecules was shut off very slowly and incompletely by bacteriophage SPO2c1. No change in the rate of incorporation of radioactive precursors into protein and ribonucleic acid (RNA) could be detected after infection, and the rate of incorporation of thymidine was increased only slightly. The relative proportions of phage and host species of nucleic acids at various intervals in the latent period were determined by means of nucleic acid hybridization. Phage-specific RNA populations synthesized early were different from those synthesized late in the latent period. Host deoxyribonucleic acid (DNA) replication continued until 8 to 10 min after SPO2c1 infection and then decreased markedly as phage-specific DNA synthesis was initiated. Host DNA was not degraded to trichloroacetic acid-soluble fragments, and its nucleotides were not found in either newly synthesized intracellular phage DNA or in progeny phage particles. The average burst size of SPO2c1 was approximately 200 plaque-forming units per cell.

FOOTNOTES

1 Present address: Department of Microbiology, The Pennsylvania State University, University Park, Pa. 16802.

J Virol. 1972 May; 9(5): 776-784
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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Copyright © 1972 by the American Society for Microbiology. All rights reserved.