This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Woodworth-Gutai, M. Articles by Levine, M. Search for Related Content PubMed PubMed Citation Articles by Woodworth-Gutai, M. Articles by Levine, M.

 Previous Article  |  Next Article 

J Virol. 1972 May; 9(5): 746-751
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

New Deoxyribonuclease Activity After Bacteriophage P22 Infection

^a Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan 48104

ABSTRACT

Extracts from P22-infected and uninfected cultures of Salmonella typhimurium were subjected to deoxyribonucleic acid (DNA)-cellulose and diethylaminoethyl-cellulose chromatography. Comparison of the elution patterns revealed that in infected cells there is a decrease in the amount of nuclease activity specific for denatured DNA and an increase in the amount of nuclease activity specific for native DNA. The latter activity was shown to differ from a similar host enzyme in Mg²⁺, Mn²⁺, and pH optima. This new activity is not found after infection of a lysogen with a nonvirulent phage or after infection under nonpermissive conditions with P22ts25.1 (a mutant in gene 25 that carries out no known functions other than adsorption and injection) and thus appears to be specified by the phage genome.

¹ Present address: Department of Cell and Molecular Biology, Medical College of Georgia, Augusta, Ga. 30902.