This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by LaMontagne, J. R. Articles by McDonald, W. C. Search for Related Content PubMed PubMed Citation Articles by LaMontagne, J. R. Articles by McDonald, W. C.

 Previous Article  |  Next Article 

J Virol. 1972 April; 9(4): 659-663
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

A Bacteriophage of Bacillus subtilis Which Forms Plaques Only at Temperatures Above 50 C III. Inhibition of TSP-1-Specific Deoxyribonucleic Acid Synthesis at 37 C and 45 C ¹

^a Department of Microbiology and Immunology, Tulane University School of Medicine, and Biology Department, Tulane University, New Orleans, Louisiana 70118

ABSTRACT

The effect of temperature on phage-specific deoxyribonucleic acid (DNA) synthesis was studied in TSP-1-infected Bacillus subtilis. This was facilitated by selectively inhibiting host DNA synthesis with 6-(p-hydroxyphenylazo)-uracil. The results indicated that TSP-1 DNA synthesis did not continue at 37 C and was immediately shut down after transfer to this temperature. Incubation at 45 C greatly reduced TSP-1 DNA synthesis. Phage-specific DNA synthesis could resume at 53 C, however, when the infected culture was returned to 53 C after a 2-min incubation period at 37 C. The results suggest that the inhibition of phage DNA synthesis at 37 C is reversible. Since infected cultures returned to 53 C after 2 min at 37 C could not complete the replicative cycle, the irreversible inhibition of yet another intermediate step was suggested.

² Present address: Department of Microbiology, School of Medicine, University of Pittsburgh, Pittsburgh, Pa. 15213.

¹ This paper was taken in part from a dissertation submitted by J. R. LaMontagne in partial fulfillment of the requirements for the Ph.D. degree at Tulane University, New Orleans, La. 70118.