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J Virol. 1972 April; 9(4): 574-582
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Specific Endonuclease R Fragments of Bacteriophage {varphi}X174 Deoxyribonucleic Acid

Marshall H. Edgell, Clyde A. Hutchison III and Morton Sclair

1 Department of Bacteriology and Immunology and Curriculum in Genetics, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27514

ABSTRACT

The restriction enzyme from Hemophilus influenzae, endonuclease R, cleaves {varphi}X174 replicative-form deoxyribonucleic acid (DNA) into at least 13 specific limit fragments. The molecular weights of 12 of the fragments have been estimated by gel electrophoresis and electron microscopy. Using the genetic assay for small fragments of {varphi}X DNA, we have shown that we can salvage markers from the endonuclease R {varphi}X-RF fragments.

J Virol. 1972 April; 9(4): 574-582
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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Copyright © 1972 by the American Society for Microbiology. All rights reserved.