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Journal of Virology, May 2009, p. 4709-4712, Vol. 83, No. 9
0022-538X/09/$08.00+0     doi:10.1128/JVI.02360-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Four Major Envelope Proteins of White Spot Syndrome Virus Bind To Form a Complex{triangledown} ,{dagger}

Qing Zhou,1 Limei Xu,2 Hui Li,2 Yi-Peng Qi,1* and Feng Yang2*

State Key Laboratory of Virology, Section of Molecular Virology, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China,1 Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, Xiamen, People's Republic of China2

Received 13 November 2008/ Accepted 4 February 2009

Early events in white spot syndrome virus (WSSV) morphogenesis, particularly the formation of viral membranes, are poorly understood. The major envelope proteins of WSSV are VP28, VP26, VP24, and VP19. Our previous results indicated that VP28 interacts with VP26 and VP24. In the present study, we used coimmunoprecipitation assays and pull-down assays to confirm that the four major proteins in the WSSV envelope can form a multiprotein complex. Yeast two-hybrid assays were also used to test for interactions among the four proteins. In summary, three pairwise protein interactions (VP19-VP28, VP19-VP24, and VP24-VP26) and one self-association (VP24-VP24) were identified for the first time.

* Corresponding author. Mailing address for F. Yang: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, 178 Daxue Rd., Xiamen 361005, People's Republic of China. Phone: 86-0592-2195274. Fax: 86-0592-2085376. E-mail: mbiotech{at}public.xm.fj.cn. Mailing address for Y.-P. Qi: State Key Laboratory of Virology, Section of Molecular Virology, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China. Phone and fax: 86-027-68754131. E-mail: qiyipeng{at}whu.edu.cn

{triangledown} Published ahead of print on 11 February 2009.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, May 2009, p. 4709-4712, Vol. 83, No. 9
0022-538X/09/$08.00+0     doi:10.1128/JVI.02360-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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