This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Junglen, S.
Right arrow Articles by Leendertz, F. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Junglen, S.
Right arrow Articles by Leendertz, F. H.

 Previous Article  |  Next Article 

Journal of Virology, May 2009, p. 4462-4468, Vol. 83, No. 9
0022-538X/09/$08.00+0     doi:10.1128/JVI.00014-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

A New Flavivirus and a New Vector: Characterization of a Novel Flavivirus Isolated from Uranotaenia Mosquitoes from a Tropical Rain Forest{triangledown} ,{dagger}

Sandra Junglen,1* Anne Kopp,1 Andreas Kurth,2 Georg Pauli,2 Heinz Ellerbrok,2 and Fabian H. Leendertz1

Research Group Emerging Zoonoses, Robert Koch Institute, Nordufer 20, D-13353 Berlin, Germany,1 Center for Biological Safety, Robert Koch Institute, Nordufer 20, D-13353 Berlin, Germany2

Received 5 January 2009/ Accepted 9 February 2009

A novel flavivirus was isolated from Uranotaenia mashonaensis, a mosquito genus not previously known to harbor flaviviruses. Mosquitoes were caught in the primary rain forest of the Taï National Park, Côte d'Ivoire. The novel virus, termed nounané virus (NOUV), seemed to grow only on C6/36 insect cells and not on vertebrate cells. Typical enveloped flavivirus-like particles of 60 to 65 nm in diameter were detected by electron microscopy in the cell culture supernatant of infected cells. The full genome was sequenced, and potential cleavage and glycosylation sites and cysteine residues were identified, suggesting that the processing of the NOUV polyprotein is similar to that of other flaviviruses. Phylogenetic analyses of the whole polyprotein and the NS3 protein showed that the virus forms a distinct cluster within the clade of mosquito-borne flaviviruses. Only a distant relationship to other known flaviviruses was found, indicating that NOUV is a novel lineage within the Flaviviridae.

* Corresponding author. Mailing address: Research Group "Emerging Zoonoses," Robert Koch Institute, Nordufer 20, D-13353 Berlin, Germany. Phone: 49 30 18754 2592. Fax: 49 30 18754 2181. E-mail: JunglenS{at}rki.de

{triangledown} Published ahead of print on 18 February 2009.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, May 2009, p. 4462-4468, Vol. 83, No. 9
0022-538X/09/$08.00+0     doi:10.1128/JVI.00014-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Huhtamo, E., Putkuri, N., Kurkela, S., Manni, T., Vaheri, A., Vapalahti, O., Uzcategui, N. Y. (2009). Characterization of a Novel Flavivirus from Mosquitoes in Northern Europe That Is Related to Mosquito-Borne Flaviviruses of the Tropics. J. Virol. 83: 9532-9540 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»