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Journal of Virology, May 2009, p. 4205-4215, Vol. 83, No. 9
0022-538X/09/$08.00+0     doi:10.1128/JVI.02403-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Isolation and Genetic Characterization of H5N2 Influenza Viruses from Pigs in Korea {triangledown}

Jun Han Lee,1 Philippe Noriel Q. Pascua,1 Min-Suk Song,1 Yun Hee Baek,1 Chul-Joong Kim,2 Hwan-Woon Choi,2,3 Moon-Hee Sung,4 Richard J. Webby,5 Robert G. Webster,5 Haryoung Poo,6 and Young Ki Choi1*

College of Medicine and Medical Research Institute, Chungbuk National University, 12 Gaeshin-Dong Heungduk-Ku, Cheongju 361-763, Republic of Korea,1 College of Veterinary Medicine, Chungnam National University, 220 Gung-Dong, Yuseoung-Gu, DaeJeon 305-764, Republic of Korea,2 Choongang Vaccine Laboratory, Daejeon 305-348, Republic of Korea,3 Bioleaders Corporation, Daejeon, Republic of Korea,4 Division of Virology, Department of Infectious Diseases, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, Tennessee 38105,5 Korean Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea6

Received 20 November 2008/ Accepted 3 February 2009

Due to dual susceptibility to both human and avian influenza A viruses, pigs are believed to be effective intermediate hosts for the spread and production of new viruses with pandemic potential. In early 2008, two swine H5N2 viruses were isolated from our routine swine surveillance in Korea. The sequencing and phylogenetic analysis of surface proteins revealed that the Sw/Korea/C12/08 and Sw/Korea/C13/08 viruses were derived from avian influenza viruses of the Eurasian lineage. However, although the Sw/Korea/C12/08 isolate is an entirely avian-like virus, the Sw/Korea/C13/08 isolate is an avian-swine-like reassortant with the PB2, PA, NP, and M genes coming from a 2006 Korean swine H3N1-like virus. The molecular characterization of the two viruses indicated an absence of significant mutations that could be associated with virulence or binding affinity. However, animal experiments showed that the reassortant Sw/Korea/C13/08 virus was more adapted and was more readily transmitted than the purely avian-like virus in a swine experimental model but not in ferrets. Furthermore, seroprevalence in swine sera from 2006 to 2008 suggested that avian H5 viruses have been infecting swine since 2006. Although there are no known potential clinical implications of the avian-swine reassortant virus for pathogenicity in pigs or other species, including humans, at present, the efficient transmissibility of the swine-adapted H5N2 virus could facilitate virus spread and could be a potential model for pandemic, highly pathogenic avian influenza (e.g., H5N1 and H7N7) virus outbreaks or a pandemic strain itself.

* Corresponding author. Mailing address: College of Medicine and Medical Research Institute, Chungbuk National University, 12 Gaeshin-Dong, Heungduk-Ku, Cheongju 361-763, Republic of Korea. Phone: 82-43-261-3384. Fax: 82-43-272-1603. E-mail: choiki55{at}chungbuk.ac.kr

{triangledown} Published ahead of print on 18 February 2009.

Journal of Virology, May 2009, p. 4205-4215, Vol. 83, No. 9
0022-538X/09/$08.00+0     doi:10.1128/JVI.02403-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Song, M.-S., Pascua, P. N. Q., Lee, J. H., Baek, Y. H., Lee, O.-J., Kim, C.-J., Kim, H., Webby, R. J., Webster, R. G., Choi, Y. K. (2009). The Polymerase Acidic Protein Gene of Influenza A Virus Contributes to Pathogenicity in a Mouse Model. J. Virol. 83: 12325-12335 [Abstract] [Full Text]  


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