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Journal of Virology, April 2009, p. 3982-3987, Vol. 83, No. 8
0022-538X/09/$08.00+0     doi:10.1128/JVI.02599-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Nipah Virus Edits Its P Gene at High Frequency To Express the V and W Proteins{triangledown}

Sachin Kulkarni,1 Valentina Volchkova,2 Christopher F. Basler,1 Peter Palese,1,3 Viktor E. Volchkov,2,4,5* and Megan L. Shaw1*

Departments of Microbiology,1 Medicine, Mount Sinai School of Medicine, New York, New York 10029,3 INSERM U758, Laboratoire des Filovirus, 21 av. Tony Garnier, Lyon F-69007, France,2 Université de Lyon, Lyon F-69007, France,4 IFR 128 BioSciences Gerland-Lyon Sud, Lyon F-69007, France5

Received 16 December 2008/ Accepted 27 January 2009

Nipah virus (NiV) is predicted to encode four proteins from its P gene (P, V, W, and C) via mRNA editing and an alternate open reading frame. By use of specific antibodies, the expression of the V, W, and C proteins in NiV-infected cells has now been confirmed. Analysis of the P-gene transcripts shows a ratio of P:V:W mRNA of 1:1:1, but this differs over time, with greater proportions of V and W transcripts observed as the infection progresses. Eighty-two percent of transcripts are edited, with up to 11 G insertions observed. This exceptionally high editing frequency ensures expression of the V and W proteins.

* Corresponding author. Mailing address for Megan L. Shaw: Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029. Phone: (212) 241-8931. Fax: (212) 534-1684. E-mail: megan.shaw{at}mssm.edu. Mailing address for Viktor E. Volchkov: Filovirus Laboratory, INSERM U758, UCBL-1, 21 av. Tony Garnier, 69365 Lyon, Cedex 07, France. Phone: 33 437282450. Fax: 33 437282459. E-mail: viktor.volchkov{at}inserm.fr

{triangledown} Published ahead of print on 11 February 2009.

Journal of Virology, April 2009, p. 3982-3987, Vol. 83, No. 8
0022-538X/09/$08.00+0     doi:10.1128/JVI.02599-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ciancanelli, M. J., Volchkova, V. A., Shaw, M. L., Volchkov, V. E., Basler, C. F. (2009). Nipah Virus Sequesters Inactive STAT1 in the Nucleus via a P Gene-Encoded Mechanism. J. Virol. 83: 7828-7841 [Abstract] [Full Text]  


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