This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bell, M. J.
Right arrow Articles by Burrows, S. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bell, M. J.
Right arrow Articles by Burrows, S. R.

 Previous Article  |  Next Article 

Journal of Virology, March 2009, p. 2783-2788, Vol. 83, No. 6
0022-538X/09/$08.00+0     doi:10.1128/JVI.01724-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

An HLA-A2-Restricted T-Cell Epitope Mapped to the BNLF2a Immune Evasion Protein of Epstein-Barr Virus That Inhibits TAP{triangledown}

Melissa J. Bell,1,{dagger} Rachel J. M. Abbott,2,{dagger} Nathan P. Croft,2 Andrew D. Hislop,2* and Scott R. Burrows1*

Queensland Institute of Medical Research and Australian Centre for Vaccine Development, Brisbane, Australia,1 Institute for Cancer Studies, University of Birmingham, Birmingham, United Kingdom2

Received 13 August 2008/ Accepted 1 January 2009

The early lytic cycle protein of Epstein-Barr virus (EBV), BNLF2a, has recently been shown to play a critical role in immune evasion by inhibiting the peptide transporter associated with antigen processing (TAP), thereby blocking antigen-specific CD8+ T-cell recognition of many lytic cycle antigens. Surprisingly, we now show that a peptide (50VLFGLLCLL58) from the hydrophobic C-terminal region of this small (60-amino-acid) EBV protein is efficiently presented by the common class I allele HLA-A2 for recognition by cytotoxic T lymphocytes. The mechanism for this unexpected finding was revealed by experiments showing that this epitope is processed and presented independently of TAP.

* Corresponding author. Mailing address for Scott R. Burrows: Queensland Institute of Medical Research, 300 Herston Rd., Brisbane 4029, Australia. Phone: 61-7-38453793. Fax: 61-7-38453510. E-mail: scottb{at}qimr.edu.au. Mailing address for Andrew D. Hislop: Institute for Cancer Studies, University of Birmingham, Birmingham, United Kingdom. Phone: 44-121-4143293. E-mail: a.d.hislop{at}bham.ac.uk

{triangledown} Published ahead of print on 7 January 2009.

{dagger} M.J.B. and R.J.M.A. contributed equally to this work.

Journal of Virology, March 2009, p. 2783-2788, Vol. 83, No. 6
0022-538X/09/$08.00+0     doi:10.1128/JVI.01724-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»