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Journal of Virology, February 2009, p. 2034-2037, Vol. 83, No. 4
0022-538X/09/$08.00+0     doi:10.1128/JVI.01883-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Replication of Equine Infectious Anemia Virus in Engineered Mouse NIH 3T3 Cells {triangledown}

Baoshan Zhang and Ronald C. Montelaro*

Center for Vaccine Research and Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261

Received 8 September 2008/ Accepted 27 November 2008

We employed the equine lentivirus equine infectious anemia virus (EIAV) to investigate the cellular restrictions for lentivirus replication in murine NIH 3T3 cells. The results of these studies demonstrate that NIH 3T3 cells expressing the EIAV receptor ELR1 and equine cyclin T1 supported productive replication of EIAV and produced infectious virions at levels similar to those found in a reference permissive equine cell line. The studies presented here demonstrate, for the first time, differential levels of restriction for EIAV and human immunodeficiency virus type 1 (HIV-1) replication in murine cells and suggest that these differences can be exploited to reveal critical virus-cell interactions required for HIV-1 assembly and budding of lentivirus particles.

* Corresponding author. Mailing address: Center for Vaccine Research, 9014 Biomedical Science Tower 3, University of Pittsburgh, Pittsburgh, PA 15261. Phone: (412) 648-8869. Fax: (412) 624-4440. E-mail: rmont{at}pitt.edu

{triangledown} Published ahead of print on 10 December 2008.

Journal of Virology, February 2009, p. 2034-2037, Vol. 83, No. 4
0022-538X/09/$08.00+0     doi:10.1128/JVI.01883-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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