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Journal of Virology, February 2009, p. 2025-2028, Vol. 83, No. 4
0022-538X/09/$08.00+0     doi:10.1128/JVI.01644-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

DNA Genome Size Affects the Stability of the Adenovirus Virion{triangledown}

Adam C. Smith,1 Kathy L. Poulin,1 and Robin J. Parks1,2*

Regenerative Medicine Program, Ottawa Health Research Institute, Ottawa, Ontario, Canada,1 Department of Biochemistry, Microbiology and Immunology, Department of Medicine, and Centre for Neuromuscular Disease, University of Ottawa, Ottawa, Ontario, Canada2

Received 1 August 2008/ Accepted 19 November 2008

Replication-defective adenovirus (Ad) vectors can vary considerably in genome length, but whether this affects virion stability has not been investigated. Helper-dependent Ad vectors with a genome size of ~30 kb were 100-fold more sensitive to heat inactivation than their parental helper virus (>36 kb), and increasing the genome size of the vector significantly improved heat stability. A similar relationship between genome size and stability existed for Ad with early region 1 deleted. Loss of infectivity was due to release of vertex proteins, followed by disintegration of the capsid. Thus, not only does the viral DNA encode all of the heritable information essential for virus replication, it also plays a critical role in maintaining capsid strength and integrity.

* Corresponding author. Mailing address: Room 4A115, 501 Smyth Road, Regenerative Medicine Program, Ottawa Health Research Institute, Ottawa, Ontario, Canada K1H 8L6. Phone: (613) 737-8123. Fax: (613) 737-8803. E-mail: rparks{at}ohri.ca

{triangledown} Published ahead of print on 26 November 2008.

Journal of Virology, February 2009, p. 2025-2028, Vol. 83, No. 4
0022-538X/09/$08.00+0     doi:10.1128/JVI.01644-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ross, P. J., Kennedy, M. A., Parks, R. J. (2009). Host Cell Detection of Noncoding Stuffer DNA Contained in Helper-Dependent Adenovirus Vectors Leads to Epigenetic Repression of Transgene Expression. J. Virol. 83: 8409-8417 [Abstract] [Full Text]  


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