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Journal of Virology, February 2009, p. 2020-2024, Vol. 83, No. 4
0022-538X/09/$08.00+0     doi:10.1128/JVI.02097-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Characterization of Neutralizing Epitopes of Varicella-Zoster Virus Glycoprotein H{triangledown}

Yasushi Akahori,1 Kazuhiro Suzuki,1,2 Tohru Daikoku,3 Masae Iwai,3 Yoshihiro Yoshida,3 Yoshizo Asano,4 Yoshikazu Kurosawa,1 and Kimiyasu Shiraki3*

Institute for Comprehensive Medical Science, Fujita Health University,1 Institute for Antibodies Ltd., Toyoake, Aichi 470-1192, Japan,2 Department of Virology, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan,3 Department of Pediatrics, School of Medicine, Fujita Health University, Toyoake, Aichi 470-1192, Japan4

Received 6 October 2008/ Accepted 30 November 2008

Varicella-zoster virus (VZV) glycoprotein H (gH) is the major neutralization target of VZV, and its neutralizing epitope is conformational. Ten neutralizing human monoclonal antibodies to gH were used to map the epitopes by immunohistochemical analysis and were categorized into seven epitope groups. The combinational neutralization efficacy of two epitope groups was not synergistic. Each epitope was partially or completely resistant to concanavalin A blocking of the glycomoiety of gH, and their antibodies inhibited the cell-to-cell spread of infection. The neutralization epitope comprised at least seven independent protein portions of gH that served as the target to inhibit cell-to-cell spread.

* Corresponding author. Mailing address: Department of Virology, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan. Phone: 81-76-434-7255. Fax: 81-76-434-5020. E-mail: kshiraki{at}med.u-toyama.ac.jp

{triangledown} Published ahead of print on 10 December 2008.

Journal of Virology, February 2009, p. 2020-2024, Vol. 83, No. 4
0022-538X/09/$08.00+0     doi:10.1128/JVI.02097-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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