This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goris, K.
Right arrow Articles by Herrler, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goris, K.
Right arrow Articles by Herrler, G.

 Previous Article  |  Next Article 

Journal of Virology, February 2009, p. 1962-1968, Vol. 83, No. 4
0022-538X/09/$08.00+0     doi:10.1128/JVI.01271-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Differential Sensitivity of Differentiated Epithelial Cells to Respiratory Viruses Reveals Different Viral Strategies of Host Infection{triangledown}

Katherina Goris,1 Sabine Uhlenbruck,1 Christel Schwegmann-Wessels,1 Wiebke Köhl,1 Frank Niedorf,2 Michael Stern,3 Marion Hewicker-Trautwein,4 Robert Bals,5 Geraldine Taylor,6 Armin Braun,7 Gerd Bicker,3 Manfred Kietzmann,2 and Georg Herrler1*

Institutes of Virology,1 Pharmacology,2 Physiology,3 Pathology, University of Veterinary Medicine Hannover, Hannover, Germany,4 Department of Internal Medicine, Division for Pulmonary Diseases, Philipps-Universität Marburg, Marburg, Germany,5 Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, United Kingdom,6 Fraunhofer Institute of Toxicology and Experimental Medicine, 30625 Hannover, Germany7

Received 19 June 2008/ Accepted 24 November 2008

To address the initiation of virus infection in the respiratory tract, we established two culture systems for differentiated bovine airway epithelial cells (BAEC). Filter-grown BAEC differentiated under air-liquid interface (ALI) conditions to generate a pseudo-stratified mucociliary epithelium. Alternatively, precision-cut lung slices (PCLS) from the bovine airways were generated that retained the original composition and distribution of differentiated epithelial cells. With both systems, epithelial cells were readily infected by bovine parainfluenza virus 3 (BPIV3). Ciliated cells were the most prominent cell type affected by BPIV3. Surprisingly, differentiated BAEC were resistant to infection by bovine respiratory syncytial virus (BRSV), when the virus was applied at the same multiplicity of infection that was sufficient for infection by BPIV3. In the case of PCLS, infection by BRSV was observed in cells located in lower cell layers but not in epithelial cells facing the lumen of the airways. The identity of the infected cells could not be determined because of a lack of specific antibodies. Increasing the virus titer 30-fold resulted in infection of the ALI cultures of BAEC, whereas in PCLS the ciliated epithelium was still refractory to infection by BRSV. These results indicate that differentiated BAEC are readily infected by BPIV3 but rather resistant to infection by BRSV. Disease caused by BRSV may require that calves encounter environmental stimuli that render BAEC susceptible to infection.

* Corresponding author. Mailing address: Institute of Virology, Stiftung Tierärztliche Hochschule Hannover, Bünteweg 17, D-30559 Hannover, Germany. Phone: 49 511 953 8857. Fax: 49 511 953 8898. E-mail: georg.herrler{at}tiho-hannover.de

{triangledown} Published ahead of print on 3 December 2008.

Journal of Virology, February 2009, p. 1962-1968, Vol. 83, No. 4
0022-538X/09/$08.00+0     doi:10.1128/JVI.01271-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»