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Journal of Virology, February 2009, p. 1660-1668, Vol. 83, No. 4
0022-538X/09/$08.00+0     doi:10.1128/JVI.01139-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Herpes Simplex Virus Replication: Roles of Viral Proteins and Nucleoporins in Capsid-Nucleus Attachment{triangledown}

Anna Maria Copeland, William W. Newcomb, and Jay C. Brown*

Department of Microbiology and Cancer Center, University of Virginia Health System, Charlottesville, Virginia 22908

Received 30 May 2008/ Accepted 3 December 2008

Replication of herpes simplex virus type 1 (HSV-1) involves a step in which a parental capsid docks onto a host nuclear pore complex (NPC). The viral genome then translocates through the nuclear pore into the nucleoplasm, where it is transcribed and replicated to propagate infection. We investigated the roles of viral and cellular proteins in the process of capsid-nucleus attachment. Vero cells were preloaded with antibodies specific for proteins of interest and infected with HSV-1 containing a green fluorescent protein-labeled capsid, and capsids bound to the nuclear surface were quantified by fluorescence microscopy. Results showed that nuclear capsid attachment was attenuated by antibodies specific for the viral tegument protein VP1/2 (UL36 gene) but not by similar antibodies specific for UL37 (a tegument protein), the major capsid protein (VP5), or VP23 (a minor capsid protein). Similar studies with antibodies specific for nucleoporins demonstrated attenuation by antibodies specific for Nup358 but not Nup214. The role of nucleoporins was further investigated with the use of small interfering RNA (siRNA). Capsid attachment to the nucleus was attenuated in cells treated with siRNA specific for either Nup214 or Nup358 but not TPR. The results are interpreted to suggest that VP1/2 is involved in specific attachment to the NPC and/or in migration of capsids to the nuclear surface. Capsids are suggested to attach to the NPC by way of the complex of Nup358 and Nup214, with high-resolution immunofluorescence studies favoring binding to Nup358.

* Corresponding author. Mailing address: Department of Microbiology, Box 800734, University of Virginia Health System, 1300 Jefferson Park Ave., Charlottesville, VA 22908. Phone: (434) 924-2504. Fax: (434) 982-1071. E-mail: jcb2g{at}virginia.edu

{triangledown} Published ahead of print on 10 December 2008.

Journal of Virology, February 2009, p. 1660-1668, Vol. 83, No. 4
0022-538X/09/$08.00+0     doi:10.1128/JVI.01139-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mohl, B. S., Bottcher, S., Granzow, H., Kuhn, J., Klupp, B. G., Mettenleiter, T. C. (2009). Intracellular Localization of the Pseudorabies Virus Large Tegument Protein pUL36. J. Virol. 83: 9641-9651 [Abstract] [Full Text]  
  • Pasdeloup, D., Blondel, D., Isidro, A. L., Rixon, F. J. (2009). Herpesvirus Capsid Association with the Nuclear Pore Complex and Viral DNA Release Involve the Nucleoporin CAN/Nup214 and the Capsid Protein pUL25. J. Virol. 83: 6610-6623 [Abstract] [Full Text]  


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