This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gammoh, N.
Right arrow Articles by Banks, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gammoh, N.
Right arrow Articles by Banks, L.

 Previous Article

Journal of Virology, February 2009, p. 1538-1543, Vol. 83, No. 3
0022-538X/09/$08.00+0     doi:10.1128/JVI.01551-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

The Mdm2 Ubiquitin Ligase Enhances Transcriptional Activity of Human Papillomavirus E2{triangledown}

Noor Gammoh, Daniela Gardiol,{dagger} Paola Massimi, and Lawrence Banks*

International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I-34012 Trieste, Italy

Received 23 July 2008/ Accepted 4 November 2008

The regulation of human papillomavirus (HPV) gene expression by the E2 protein is a critical feature of the viral life cycle. Previous studies have shown an important role in transcription for the ubiquitin-proteasome pathway, but its role in HPV gene expression has not been addressed. We now show that HPV E2 requires an active proteasome for its optimal transcriptional activator function. This involves an interaction with the Mdm2 ubiquitin ligase, which together with E2 acts synergistically to activate the HPV type 16 promoter. We also show that HPV E2 recruits Mdm2 onto HPV promoter sequences, providing an explanation for this cooperative activity.

* Corresponding author. Mailing address: Tumour Virology Lab, ICGEB, Padriciano 99, 34012 Trieste, Italy. Phone: 39 040 3757328. Fax: 39 040 226555. E-mail: banks{at}icgeb.org

{triangledown} Published ahead of print on 12 November 2008.

{dagger} Present address: IBR/CONICET, Facultad de Bioquímica, Suipacha 531 (2000), Rosario, Argentina.

Journal of Virology, February 2009, p. 1538-1543, Vol. 83, No. 3
0022-538X/09/$08.00+0     doi:10.1128/JVI.01551-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gagnon, D., Joubert, S., Senechal, H., Fradet-Turcotte, A., Torre, S., Archambault, J. (2009). Proteasomal Degradation of the Papillomavirus E2 Protein Is Inhibited by Overexpression of Bromodomain-Containing Protein 4. J. Virol. 83: 4127-4139 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»