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Journal of Virology, December 2009, p. 12545-12551, Vol. 83, No. 23
0022-538X/09/$08.00+0     doi:10.1128/JVI.01624-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Distinct Regulation of Hepatitis B Virus Biosynthesis by Peroxisome Proliferator-Activated Receptor {gamma} Coactivator 1{alpha} and Small Heterodimer Partner in Human Hepatoma Cell Lines{triangledown}

Caitlin R. Ondracek,{dagger} Vanessa C. Reese,{dagger} Christel N. Rushing, Claudia E. Oropeza, and Alan McLachlan*

Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, 909 South Wolcott Avenue, Chicago, Illinois 60612

Received 4 August 2009/ Accepted 21 September 2009

The human hepatoma cell lines HepG2 and Huh7 have been used extensively to study hepatitis B virus (HBV) transcription and replication. Both cell lines support transcription of the 3.5-kb viral pregenomic RNA and subsequent viral DNA synthesis by reverse transcription. The effects of the coactivator peroxisome proliferator-activated receptor {gamma} coactivator 1{alpha} (PGC1{alpha}) and corepressor small heterodimer partner (SHP) on HBV transcription and replication mediated by nuclear receptors were examined in the context of individual nuclear receptors in nonhepatoma cells and in hepatoma cells in an attempt to determine the relative contribution of the various nuclear receptors to viral biosynthesis in the hepatoma cells. PGC1{alpha} and SHP modulated viral biosynthesis differently in the human hepatoma cell lines HepG2 and Huh7, indicating distinct modes of transcriptional regulation. Consistent with this suggestion, it appears that retinoid X receptor {alpha}/farnesoid X receptor {alpha} and liver receptor homolog 1 or estrogen-related receptor β (ERRβ) may contribute to the majority of the viral replication observed in HepG2 cells, whereas ERR{alpha} and ERR{gamma} are probably responsible for the majority of viral biosynthesis in Huh7 cells. Therefore, this approach indicates that the transcriptional regulation of HBV biosynthesis in HepG2 and Huh7 cells is primarily controlled by different transcription factors.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, 909 South Wolcott Avenue, Chicago, IL 60612. Phone: (312) 355-0211. Fax: (312) 996-4890. E-mail: mclach{at}uic.edu

{triangledown} Published ahead of print on 30 September 2009.

{dagger} C.R.O. and V.C.R. contributed equally to this study.

Journal of Virology, December 2009, p. 12545-12551, Vol. 83, No. 23
0022-538X/09/$08.00+0     doi:10.1128/JVI.01624-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ondracek, C. R., Rushing, C. N., Reese, V. C., Oropeza, C. E., McLachlan, A. (2009). Peroxisome Proliferator-Activated Receptor {gamma} Coactivator 1{alpha} and Small Heterodimer Partner Differentially Regulate Nuclear Receptor-Dependent Hepatitis B Virus Biosynthesis. J. Virol. 83: 12535-12544 [Abstract] [Full Text]  


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