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Journal of Virology, November 2009, p. 11402-11406, Vol. 83, No. 21
0022-538X/09/$08.00+0     doi:10.1128/JVI.00504-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Characterization of a Mumps Virus Nucleocapsidlike Particle {triangledown}

Robert Cox,1 Todd J. Green,1 Shihong Qiu,1 Jungsoon Kang,1 Jun Tsao,1 Peter E. Prevelige,1 Biao He,2 and Ming Luo1*

Department of Microbiology, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, Alabama 35294,1 Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, Pennsylvania 168022

Received 11 March 2009/ Accepted 7 August 2009

The nucleocapsid protein (NP) of mumps virus (MuV), a paramyxovirus, was coexpressed with the phosphoprotein (P) in Escherichia coli. The NP and P proteins form a soluble complex containing RNA. Under a transmission electron microscope, the NP-RNA complex appears as a nucleocapsidlike ring that has a diameter of approximately 20 nm with 13 subunits. There is a piece of single-stranded RNA with a length of 78 nucleotides in the NP-RNA ring. Shorter RNA pieces are also visible. The MuV NP protein may provide weaker protection of the RNA than the NP protein of some other negative-strand RNA viruses, reflecting the degree of NP protein association.

* Corresponding author. Mailing address: Room 111, CBSE, 1025 18th Street South, University of Alabama at Birmingham, Birmingham, AL 35294. Phone: (205) 934-4259. Fax: (205) 975-9578. E-mail: mingluo{at}uab.edu

{triangledown} Published ahead of print on 19 August 2009.

Journal of Virology, November 2009, p. 11402-11406, Vol. 83, No. 21
0022-538X/09/$08.00+0     doi:10.1128/JVI.00504-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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