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Journal of Virology, November 2009, p. 11372-11377, Vol. 83, No. 21
0022-538X/09/$08.00+0     doi:10.1128/JVI.01228-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

VP5* Rearranges when Rotavirus Uncoats {triangledown}

Joshua D. Yoder,1,{dagger} Shane D. Trask,1,{ddagger} Phuoc T. Vo,2,3 Mawuena Binka,1,§ Ningguo Feng,2,3 Stephen C. Harrison,1,4 Harry B. Greenberg,2 and Philip R. Dormitzer1*

Laboratory of Molecular Medicine, Children's Hospital, Boston, Massachusetts 02115,1 Department of Microbiology and Immunology and Department of Medicine, Stanford University School of Medicine, Stanford, California 94305,2 Palo Alto VA Health Care System, Palo Alto, California 94304,3 the Howard Hughes Medical Institute, Children's Hospital, Boston, Massachusetts 021154

Received 14 June 2009/ Accepted 11 August 2009

Trypsin primes rotavirus for efficient infectivity by cleaving the spike protein, VP4, into VP8* and VP5*. A recombinant VP5* fragment has a trimeric, folded-back structure. Comparison of this structure with virion spikes suggests that a rearrangement, analogous to those of enveloped virus fusion proteins, may mediate membrane penetration by rotavirus during entry. To detect this inferred rearrangement of virion-associated authentic VP5*, we raised conformation-specific monoclonal antibodies against the recombinant VP5* fragment in its putative post-membrane penetration conformation. Using one of these antibodies, we demonstrate that rotavirus uncoating triggers a conformational change in the cleaved VP4 spike to yield rearranged VP5*.

* Corresponding author. Present address: Novartis Vaccines and Diagnostics, Inc., 350 Massachusetts Ave., Cambridge, MA 02139. Phone: (617) 230-2459. Fax: (617) 871-8911. E-mail: philip.dormitzer{at}novartis.com

{triangledown} Published ahead of print on 19 August 2009.

{dagger} Present address: Purdue University, West Lafayette, IN 47907.

{ddagger} Present address: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-8026.

§ Present address: Graduate School of Biological Sciences, Mount Sinai School of Medicine, New York, NY 10029.

Journal of Virology, November 2009, p. 11372-11377, Vol. 83, No. 21
0022-538X/09/$08.00+0     doi:10.1128/JVI.01228-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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