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Journal of Virology, October 2009, p. 10836-10839, Vol. 83, No. 20
0022-538X/09/$08.00+0     doi:10.1128/JVI.00998-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification of a Novel Astrovirus (Astrovirus VA1) Associated with an Outbreak of Acute Gastroenteritis{triangledown}

Stacy R. Finkbeiner,1,{dagger} Yan Li,2,{dagger} Susan Ruone,2 Christina Conrardy,2 Nicole Gregoricus,2 Denise Toney,3 Herbert W. Virgin,1 Larry J. Anderson,2 Jan Vinjé,2 David Wang,1* and Suxiang Tong2*

Departments of Molecular Microbiology and Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110,1 Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,2 Commonwealth of Virginia Division of Consolidated Laboratory Services, Richmond, Virginia 232193

Received 17 May 2009/ Accepted 31 July 2009

The etiology of a large proportion of gastrointestinal illness is unknown. In this study, random Sanger sequencing and pyrosequencing approaches were used to analyze fecal specimens from a gastroenteritis outbreak of unknown etiology in a child care center. Multiple sequences with limited identity to known astroviruses were identified. Assembly of the sequences and subsequent reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends generated a complete genome of 6,586 nucleotides. Phylogenetic analysis demonstrated that this virus, named astrovirus VA1 (AstV-VA1), is highly divergent from all previously described astroviruses. Based on RT-PCR, specimens from multiple patients in this outbreak were unequivocally positive for Ast-VA1.

* Corresponding author. Mailing address for David Wang: Departments of Molecular Microbiology and Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110. Phone: (314) 286-1123. Fax: (314) 362-7325. E-mail: davewang{at}borcim.wustl.edu. Mailing address for Suxiang Tong: Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333. Phone: (404) 639-1372. Fax: (404) 639-4005. E-mail: stong{at}cdc.gov

{triangledown} Published ahead of print on 12 August 2009.

{dagger} These authors contributed equally to the work.

Journal of Virology, October 2009, p. 10836-10839, Vol. 83, No. 20
0022-538X/09/$08.00+0     doi:10.1128/JVI.00998-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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