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Journal of Virology, October 2009, p. 10830-10835, Vol. 83, No. 20
0022-538X/09/$08.00+0     doi:10.1128/JVI.01067-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Association of Rice Gall Dwarf Virus with Microtubules Is Necessary for Viral Release from Cultured Insect Vector Cells{triangledown}

Taiyun Wei,1,{dagger} Tamaki Uehara-Ichiki,1,{dagger} Naoyuki Miyazaki,2 Hiroyuki Hibino,1 Kenji Iwasaki,2 and Toshihiro Omura1*

National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666,1 Institute for Protein Research, Osaka University, CREST, JST, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan2

Received 25 May 2009/ Accepted 23 July 2009

Vector insect cells infected with Rice gall dwarf virus, a member of the family Reoviridae, contained the virus-associated microtubules adjacent to the viroplasms, as revealed by transmission electron, electron tomographic, and confocal microscopy. The viroplasms, putative sites of viral replication, contained the nonstructural viral proteins Pns7 and Pns12, as well as core protein P5, of the virus. Microtubule-depolymerizing drugs suppressed the association of viral particles with microtubules and prevented the release of viruses from cells without significantly affecting viral multiplication. Thus, microtubules appear to mediate viral transport within and release of viruses from infected vector cells.

* Corresponding author. Mailing address: National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan. Phone: 81-29-838-8932. Fax: 81-29-838-7845. E-mail: toomura{at}affrc.go.jp

{triangledown} Published ahead of print on 29 July 2009.

{dagger} These authors contributed equally to this work.

Journal of Virology, October 2009, p. 10830-10835, Vol. 83, No. 20
0022-538X/09/$08.00+0     doi:10.1128/JVI.01067-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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