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Journal of Virology, January 2009, p. 633-639, Vol. 83, No. 2
0022-538X/09/$08.00+0     doi:10.1128/JVI.01766-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Temporal Control of Message Stability in the Life Cycle of Double-Stranded RNA Bacteriophage {Phi}8{triangledown}

Xueying Qiao, Yang Sun, Jian Qiao, and Leonard Mindich*

Department of Microbiology, the Public Health Research Institute, Newark, New Jersey 07103

Received 21 August 2008/ Accepted 16 October 2008

The cystoviruses have genomes of three double-stranded RNA segments. The genes of the L transcript are expressed early in infection, while those of M and S are expressed late. In all cystovirus groups but one, the quantity of the L transcript late in infection is lower than those of the other two because of transcriptional control. In bacteriophage {Phi}8 and its close relatives, transcription of L is not controlled; instead, the L transcript is turned over rapidly late in infection. The three messages are produced in approximately equal amounts early in infection, but the amount of L is less than 10% of the amounts of the others late in infection. The decay of the {Phi}8 L message depends upon the production of protein Hb, which is encoded in segment L. It also depends upon a target site within the H gene region. Phage mutants lacking either the Hb gene or the target region do not show the late control of L message quantity. In addition to having a role as a negative regulator, Hb functions to neutralize the activity of protein J, encoded by segment S, which causes the degradation of all viral transcripts.

* Corresponding author. Mailing address: Public Health Research Institute Center at NJMS, 225 Warren Street, Newark, NJ 07103. Phone: (973) 854-3420. Fax: (973) 854-3453. E-mail: mindicle{at}umdnj.edu

{triangledown} Published ahead of print on 29 October 2008.

Journal of Virology, January 2009, p. 633-639, Vol. 83, No. 2
0022-538X/09/$08.00+0     doi:10.1128/JVI.01766-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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