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 Previous Article

Journal of Virology, January 2009, p. 1161-1166, Vol. 83, No. 2
0022-538X/09/$08.00+0     doi:10.1128/JVI.01362-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Conformational Changes in the Solution Structure of the Dengue Virus 5' End in the Presence and Absence of the 3' Untranslated Region{triangledown}

Charlotta Polacek, Jonathan E. Foley, and Eva Harris*

Division of Infectious Diseases, School of Public Health, University of California, Berkeley, 1 Barker Hall, Berkeley, California 94720-7354

Received 30 June 2008/ Accepted 31 October 2008

Dengue virus (DENV) is an ~10.7-kb positive-sense RNA virus that circularizes via RNA-RNA interactions between sequences in the 5' and 3' terminal regions. Complementarity between the cyclization sequence (CS) and the upstream AUG region (UAR) has been shown to be necessary for viral replication. Here, we present the solution structure of the 5' end of DENV type 2 in the presence and absence of the 3' end. We demonstrate that hybridization between the 5' and 3' CSs is independent of the UAR while the 5' UAR-3' UAR hybridization is dependent upon the 5' CS-3' CS interaction.

* Corresponding author. Mailing address: Division of Infectious Diseases, School of Public Health, University of California, Berkeley, 1 Barker Hall, Berkeley, CA 94720-7354. Phone: (510) 642-4845. Fax: (510) 642-6350. E-mail: eharris{at}berkeley.edu

{triangledown} Published ahead of print on 12 November 2008.

Journal of Virology, January 2009, p. 1161-1166, Vol. 83, No. 2
0022-538X/09/$08.00+0     doi:10.1128/JVI.01362-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Romero-Lopez, C., Berzal-Herranz, A. (2009). A long-range RNA-RNA interaction between the 5' and 3' ends of the HCV genome. RNA 15: 1740-1752 [Abstract] [Full Text]  


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