This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reina, R.
Right arrow Articles by Rosati, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reina, R.
Right arrow Articles by Rosati, S.

 Previous Article  |  Next Article 

Journal of Virology, January 2009, p. 1152-1155, Vol. 83, No. 2
0022-538X/09/$08.00+0     doi:10.1128/JVI.01627-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Genome Analysis of Small-Ruminant Lentivirus Genotype E: a Caprine Lentivirus with Natural Deletions of the dUTPase Subunit, vpr-Like Accessory Gene, and 70-Base-Pair Repeat of the U3 Region {triangledown}

Ramsés Reina,{dagger} Elena Grego,{dagger} Luigi Bertolotti, Daniele De Meneghi, and Sergio Rosati*

Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università degli Studi di Torino, Grugliasco, Italy

Received 30 July 2008/ Accepted 24 October 2008

The nucleotide sequence of the highly divergent small-ruminant lentivirus genotype E has been determined. The full genome consists of 8,418 nucleotides and lacks two large portions corresponding nearly to the entire dUTPase subunit of the pol and vpr-like accessory genes. Moreover, the 70-bp repeat of the U3 region of the long terminal repeat was observed to be deleted. Interestingly, this lentivirus genotype is able to persist in a local breed population, and retrospective analysis revealed its presence in milk samples collected in 1999. gag sequences obtained from a flock coinfected with the B1 and E genotypes revealed that the evolutionary rates of the two viruses were quite similar. Since a reduced viral load and/or disease progression was observed for viruses with artificially deleted dUTPase and vpr-like genes, it is proposed that this viral cluster be designated a low-pathogenicity caprine lentivirus.

* Corresponding author. Mailing address: Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Via Leonardo da Vinci, 44, 10095 Grugliasco (TO), Italy. Phone: 39011 6709187. Fax: 390116709196. E-mail: sergio.rosati{at}unito.it

{triangledown} Published ahead of print on 5 November 2008.

{dagger} Both authors contributed equally to this work.

Journal of Virology, January 2009, p. 1152-1155, Vol. 83, No. 2
0022-538X/09/$08.00+0     doi:10.1128/JVI.01627-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»