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Journal of Virology, October 2009, p. 9682-9693, Vol. 83, No. 19
0022-538X/09/$08.00+0     doi:10.1128/JVI.00715-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Respiratory Syncytial Virus Nonstructural Proteins Decrease Levels of Multiple Members of the Cellular Interferon Pathways{triangledown}

Samer Swedan, Alla Musiyenko, and Sailen Barik*

Department of Biochemistry and Molecular Biology, University of South Alabama, College of Medicine, 307 University Blvd., Mobile, Alabama 36688-0002

Received 6 April 2009/ Accepted 17 July 2009

Viruses of the Paramyxoviridae family, such as the respiratory syncytial virus (RSV), suppress cellular innate immunity represented by type I interferon (IFN) for optimal growth in their hosts. The two unique nonstructural (NS) proteins, NS1 and NS2, of RSV suppress IFN synthesis, as well as IFN function, but their exact targets are still uncharacterized. Here, we investigate if either or both of the NS proteins affect the steady-state levels of key members of the IFN pathway. We found that both NS1 and NS2 decreased the levels of TRAF3, a strategic integrator of multiple IFN-inducing signals, although NS1 was more efficient. Only NS1 reduced IKK{varepsilon}, a key protein kinase that specifically phosphorylates and activates IFN regulatory factor 3. Loss of the TRAF3 and IKK{varepsilon} proteins appeared to involve a nonproteasomal mechanism. Interestingly, NS2 modestly increased IKK{varepsilon} levels. In the IFN response pathway, NS2 decreased the levels of STAT2, the essential transcription factor for IFN-inducible antiviral genes. Preliminary mapping revealed that the C-terminal 10 residues of NS1 were essential for reducing IKK{varepsilon} levels and the C-terminal 10 residues of NS2 were essential for increasing and reducing IKK{varepsilon} and STAT2, respectively. In contrast, deletion of up to 20 residues of the C termini of NS1 and NS2 did not diminish their TRAF3-reducing activity. Coimmunoprecipitation studies revealed that NS1 and NS2 form a heterodimer. Clearly, the NS proteins of RSV, working individually and together, regulate key signaling molecules of both the IFN activation and response pathways.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, University of South Alabama, College of Medicine, 307 University Blvd., Mobile, AL 36688-0002. Phone: (251) 460-6860. Fax: (251) 460-6850. E-mail: sbarik{at}jaguar1.usouthal.edu

{triangledown} Published ahead of print on 22 July 2009.

Journal of Virology, October 2009, p. 9682-9693, Vol. 83, No. 19
0022-538X/09/$08.00+0     doi:10.1128/JVI.00715-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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