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Journal of Virology, October 2009, p. 10264-10268, Vol. 83, No. 19
0022-538X/09/$08.00+0     doi:10.1128/JVI.01255-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

The Choice of Translation Initiation Site of the Rep Proteins from Goose Parvovirus P9-Generated mRNA Is Governed by Splicing and the Nature of the Excised Intron{triangledown}

Long Li,1 Jianming Qiu,2 and David J. Pintel1*

Department of Molecular Microbiology and Immunology, University of Missouri-Columbia, School of Medicine, Bond Life Sciences Center, Columbia, Missouri,1 Department of Microbiology, Immunology, and Molecular Genetics, University of Kansas Medical Center, Kansas City, Kansas2

Received 17 June 2009/ Accepted 10 July 2009

The goose parvovirus (GPV) Rep 1 and Rep 2 proteins are encoded by P9-generated mRNAs that are either unspliced or spliced within the rep gene region, respectively. These mRNAs are present in an approximately equal ratio. The translation of Rep 1 was initiated from the first AUG in unspliced P9-generated mRNA; however, this AUG was bypassed in spliced P9-generated RNA and Rep 2 translation initiated predominately at the next initiating AUG downstream. We show that the choice of the site of initiation of translation of GPV Rep-encoding mRNAs is governed both by the splicing process itself and by the nature of the excised intron.

* Corresponding author. Mailing address: 471f Life Sciences Center, University of Missouri-Columbia, 1201 Rollins Rd., Columbia, MO 65211. Phone: (573) 882-3920. Fax: (573) 884-9676. E-mail: pinteld{at}missouri.edu

{triangledown} Published ahead of print on 22 July 2009.

Journal of Virology, October 2009, p. 10264-10268, Vol. 83, No. 19
0022-538X/09/$08.00+0     doi:10.1128/JVI.01255-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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