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Journal of Virology, October 2009, p. 10256-10263, Vol. 83, No. 19
0022-538X/09/$08.00+0     doi:10.1128/JVI.02654-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Human Immunodeficiency Virus Type 1 Vpr Modulates Cellular Expression of UNG2 via a Negative Transcriptional Effect{triangledown}

Christelle Langevin,1,2 Priscilla Maidou-Peindara,1,2 Per Arne Aas,3 Guillaume Jacquot,1,2 Marit Otterlei,3 Geir Slupphaug,3 and Serge Benichou1,2*

Institut Cochin, CNRS UMR 8104, Université Paris Descartes, Paris, France,1 INSERM U567, Paris, France,2 Department of Cancer Research and Molecular Medicine, Norvegian University of Science and Technology, Trondheim, Norway3

Received 24 December 2008/ Accepted 13 July 2009

It was recently reported that human immunodeficiency virus type 1 (HIV-1) Vpr induced the proteasomal degradation of the nuclear UNG2 enzyme for efficient virus replication. We confirm here that HIV-1 infection and Vpr expression reduce the level of endogenous UNG2, but this effect is not reverted by treatment with the proteasome inhibitor MG132. Moreover, this reduction is not mediated by Vpr binding to UNG2 and is independent of the Vpr-induced G2 arrest. Finally, we show that Vpr influences the UNG2 promoter without affecting UNG1 gene expression. These data indicate that the Vpr-induced decrease of UNG2 level is mainly related to a transcriptional effect.

* Corresponding author. Mailing address: Institut Cochin, 27 Rue du Faubourg Saint-Jacques, 75014 Paris, France. Phone: (33) 1 40 51 65 78. Fax: (33) 1 40 51 65 70. E-mail: serge.benichou{at}inserm.fr

{triangledown} Published ahead of print on 22 July 2009.

Journal of Virology, October 2009, p. 10256-10263, Vol. 83, No. 19
0022-538X/09/$08.00+0     doi:10.1128/JVI.02654-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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