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Journal of Virology, October 2009, p. 10250-10255, Vol. 83, No. 19
0022-538X/09/$08.00+0     doi:10.1128/JVI.01038-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Role of the Cytoplasmic Tail Amino Acid Sequences of Newcastle Disease Virus Hemagglutinin-Neuraminidase Protein in Virion Incorporation, Cell Fusion, and Pathogenicity {triangledown}

Shin-Hee Kim, Yongqi Yan, and Siba K. Samal*

Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, 8075 Greenmead Drive, College Park, Maryland 20742

Received 21 May 2009/ Accepted 10 July 2009

To determine the role of amino acid sequences of the hemagglutinin-neuraminidase (HN) cytoplasmic tail in Newcastle disease virus (NDV) replication and pathogenicity, we generated recombinant NDVs with a deletion or point mutation in the N-terminal cytoplasmic tail. The first 2-amino-acid deletion in the cytoplasmic tail did not affect the biological characteristics of NDV. However, a 4-amino-acid deletion and the substitution of alanine for serine at position 6 affected cell fusion, pathogenicity, and colocalization of the HN and M proteins of NDV, indicating that these residues of the HN cytoplasmic tail are critical for its specific incorporation into virions.

* Corresponding author. Mailing address: Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, 8075 Greenmead Drive, College Park, MD 20742. Phone: (301) 314-6813. Fax: (301) 314-6855. E-mail: ssamal{at}umd.edu

{triangledown} Published ahead of print on 29 July 2009.

Journal of Virology, October 2009, p. 10250-10255, Vol. 83, No. 19
0022-538X/09/$08.00+0     doi:10.1128/JVI.01038-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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