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Journal of Virology, September 2009, p. 9591-9595, Vol. 83, No. 18
0022-538X/09/$08.00+0     doi:10.1128/JVI.01115-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Recruitment of the Transcriptional Coactivator HCF-1 to Viral Immediate-Early Promoters during Initiation of Reactivation from Latency of Herpes Simplex Virus Type 1{triangledown} ,{dagger}

Zackary W. Whitlow and Thomas M. Kristie*

Molecular Genetics Section, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

Received 1 June 2009/ Accepted 23 June 2009

The transcriptional coactivator host cell factor 1 (HCF-1) is critical for the expression of immediate-early (IE) genes of the alphaherpesviruses herpes simplex virus type 1 (HSV-1) and varicella-zoster virus. HCF-1 may also be involved in the reactivation of these viruses from latency as it is sequestered in the cytoplasm of sensory neurons but is rapidly relocalized to the nucleus upon stimulation that results in reactivation. Here, chromatin immunoprecipitation assays demonstrate that HCF-1 is recruited to IE promoters of viral genomes during the initiation of reactivation, correlating with RNA polymerase II occupancy and IE expression. The data support the model whereby HCF-1 plays a pivotal role in the reactivation of HSV-1 from latency.

* Corresponding author. Mailing address: Laboratory of Viral Diseases, National Institutes of Health, Building 4, Room 129, 4 Center Drive, Bethesda, MD 20892. Phone: (301) 496-3854. Fax: (301) 480-1560.E-mail: Thomas_kristie{at}nih.gov

{triangledown} Published ahead of print on 1 July 2009.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, September 2009, p. 9591-9595, Vol. 83, No. 18
0022-538X/09/$08.00+0     doi:10.1128/JVI.01115-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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