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Journal of Virology, September 2009, p. 9002-9007, Vol. 83, No. 17
0022-538X/09/$08.00+0     doi:10.1128/JVI.00423-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

The Infectious Molecular Clone and Pseudotyped Virus Models of Human Immunodeficiency Virus Type 1 Exhibit Significant Differences in Virion Composition with Only Moderate Differences in Infectivity and Inhibition Sensitivity {triangledown}

Nicholas M. Provine,1,{dagger} Wendy Blay Puryear,2,{dagger} Xueling Wu,3 Julie Overbaugh,3 and Nancy L. Haigwood4*

Department of Microbiology, University of Washington, Seattle, Washington 98195,1 Seattle Biomedical Research Institute, Seattle, Washington 98109,2 Divisions of Human Biology and Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104,3 Division of Pathology and Immunology, Oregon National Primate Research Center, Beaverton, Oregon 970064

Received 26 February 2009/ Accepted 11 June 2009

Two frequently employed methods for generating well-characterized, genetically defined infectious human immunodeficiency virus type 1 in vitro include the use of infectious molecular clones (IMCs) and pseudoviruses (PVs) competent for single-round infection. We compared six matched pairs of IMCs and PVs. The relative amounts of Env incorporated and efficiency of cleavage differed substantially between the two systems. Altering the ratio of proviral genome and env expression plasmids can produce pseudovirions that are structurally more similar to the matched IMCs. Differences in Env incorporation and cleavage translated into moderate differences in assays infectivity and sensitivity to neutralizing antibodies and entry inhibitors.

* Corresponding author. Mailing address: Oregon National Primate Research Center, Beaverton, OR 97006. Phone: (503) 690-5500. Fax: (503) 690-5569. E-mail: haigwoon{at}ohsu.edu

{triangledown} Published ahead of print on 17 June 2009.

{dagger} These authors contributed equally to this work.

Journal of Virology, September 2009, p. 9002-9007, Vol. 83, No. 17
0022-538X/09/$08.00+0     doi:10.1128/JVI.00423-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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