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Journal of Virology, September 2009, p. 8986-8992, Vol. 83, No. 17
0022-538X/09/$08.00+0     doi:10.1128/JVI.00599-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Balancing Reversion of Cytotoxic T-Lymphocyte and Neutralizing Antibody Escape Mutations within Human Immunodeficiency Virus Type 1 Env upon Transmission{triangledown} ,{dagger}

Viv Peut,1 Shahan Campbell,1 Adriana Gaeguta,1 Rob J. Center,1 Kim Wilson,2 Sheilajen Alcantara,1 Caroline S. Fernandez,1 Damian F. J. Purcell,1 and Stephen J. Kent1*

Department of Microbiology and Immunology, University of Melbourne, Melbourne, Australia,1 National Serology Reference Laboratory, St. Vincent's Institute, Melbourne, Australia2

Received 23 March 2009/ Accepted 4 June 2009

Human immunodeficiency virus type 1 (HIV-1) envelope protein (Env) is subject to both neutralizing antibody (NAb) and CD8 T-cell (cytotoxic T-lymphocyte [CTL]) immune pressure. We studied the reversion of the Env CTL escape mutant virus to the wild type and the relationship between the reversion of CTL mutations with N-linked glycosylation site (NLGS)-driven NAb escape in pigtailed macaques. Env CTL mutations either did not revert to the wild type or only transiently reverted 5 to 7 weeks after infection. The CTL escape mutant reversion was coincident, for the same viral clones, with the loss of NLGS mutations. At one site studied, both CTL and NLGS mutations were needed to confer NAb escape. We conclude that CTL and NAb escape within Env can be tightly linked, suggesting opportunities to induce effective multicomponent anti-Env immunity.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Melbourne, Melbourne, Australia. Phone: 61-383449939. Fax: 61-383443846. E-mail: skent{at}unimelb.edu.au

{triangledown} Published ahead of print on 10 June 2009.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, September 2009, p. 8986-8992, Vol. 83, No. 17
0022-538X/09/$08.00+0     doi:10.1128/JVI.00599-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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