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Journal of Virology, September 2009, p. 8976-8979, Vol. 83, No. 17
0022-538X/09/$08.00+0     doi:10.1128/JVI.00142-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Cell Fusion-Induced Activation of Interferon-Stimulated Genes Is Not Required for Restriction of a Herpes Simplex Virus VP16/ICP0 Mutant in Heterokarya Formed between Permissive and Restrictive Cells{triangledown}

Meaghan H. Hancock,1 Karen L. Mossman,2 and James R. Smiley1*

Alberta Institute for Viral Immunology, Department of Medical Microbiology & Immunology, University of Alberta, Edmonton, Alberta T6G 2S2, Canada,1 Department of Pathology & Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada2

Received 21 January 2009/ Accepted 3 June 2009

Herpes simplex virus VP16 and ICP0 mutants replicate efficiently in U2OS osteosarcoma cells but are restricted in other cell types. We previously showed that the restrictive phenotype is dominant in a transient cell fusion assay, suggesting that U2OS cells lack an antiviral mechanism present in other cells. Recent data indicate that unscheduled membrane fusion events can activate the expression of interferon-stimulated genes (ISGs) in fibroblasts, raising the possibility that our earlier results were due to a fusion-induced antiviral state. However, we show here that the permissive phenotype is also extinguished following fusion with Vero cells in the absence of ISG induction.

* Corresponding author. Mailing address: Alberta Institute for Viral Immunology, Department of Medical Microbiology & Immunology, 632 Heritage Medical Research Center, University of Alberta, Edmonton, Alberta T6G 2S2, Canada. Phone: (780) 492-4070. Fax: (780) 492-9828. E-mail: jim.smiley{at}ualberta.ca

{triangledown} Published ahead of print on 17 June 2009.

Journal of Virology, September 2009, p. 8976-8979, Vol. 83, No. 17
0022-538X/09/$08.00+0     doi:10.1128/JVI.00142-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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