This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Souki, S. K.
Right arrow Articles by Sandri-Goldin, R. M.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Souki, S. K.
Right arrow Articles by Sandri-Goldin, R. M.

 Previous Article  |  Next Article 

Journal of Virology, September 2009, p. 8970-8975, Vol. 83, No. 17
0022-538X/09/$08.00+0     doi:10.1128/JVI.00801-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Arginine Methylation of the ICP27 RGG Box Regulates the Functional Interactions of ICP27 with SRPK1 and Aly/REF during Herpes Simplex Virus 1 Infection {triangledown}

Stuart K. Souki and Rozanne M. Sandri-Goldin*

Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, California 92697

Received 20 April 2009/ Accepted 16 June 2009

The herpes simplex virus 1 protein ICP27 is methylated on arginine residues within an RGG box, and arginine methylation regulates ICP27 export to the cytoplasm. Arginine methylation can regulate protein-protein interactions; therefore, we examined the effect of hypomethylation on ICP27's interactions with cellular proteins SRPK1 and Aly/REF, which bind to ICP27 through the RGG box region. During infections with viral mutants containing lysine substitutions or the methylation inhibitor adenosine dialdehyde, the interaction of ICP27 with SRPK1 and Aly/REF was decreased, as determined by coimmunoprecipitation and colocalization studies, indicating that ICP27 RGG box methylation regulates interaction with these proteins.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, School of Medicine, Medical Sciences, B240, University of California, Irvine, CA 92697-4025. Phone: (949) 824-7570. Fax: (949) 824-9054. E-mail: rmsandri{at}uci.edu

{triangledown} Published ahead of print on 24 June 2009.

Journal of Virology, September 2009, p. 8970-8975, Vol. 83, No. 17
0022-538X/09/$08.00+0     doi:10.1128/JVI.00801-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»