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Journal of Virology, September 2009, p. 8428-8438, Vol. 83, No. 17
0022-538X/09/$08.00+0     doi:10.1128/JVI.00659-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

A Novel Genotype H9N2 Influenza Virus Possessing Human H5N1 Internal Genomes Has Been Circulating in Poultry in Eastern China since 1998 ^,

Pinghu Zhang,^1,2 Yinghua Tang,¹ Xiaowen Liu,¹ Wenbo Liu,¹ Xiaorong Zhang,¹ Hongqi Liu,^1, Daxin Peng,¹ Song Gao,¹ Yantao Wu,¹ Luyong Zhang,² Shan Lu,^3,4 and Xiufan Liu^1*

Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China,¹ National Drug Screening Laboratory, New Drug Screening Center, China Pharmaceutical University, Nanjing 210009, China,² China-US Vaccine Research Center, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China,³ Laboratory of Nucleic Acid Vaccines, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605⁴

Received 30 March 2009/ Accepted 5 June 2009

Many novel reassortant influenza viruses of the H9N2 genotype have emerged in aquatic birds in southern China since their initial isolation in this region in 1994. However, the genesis and evolution of H9N2 viruses in poultry in eastern China have not been investigated systematically. In the current study, H9N2 influenza viruses isolated from poultry in eastern China during the past 10 years were characterized genetically and antigenically. Phylogenetic analysis revealed that these H9N2 viruses have undergone extensive reassortment to generate multiple novel genotypes, including four genotypes (J, F, K, and L) that have never been recognized before. The major H9N2 influenza viruses represented by A/Chicken/Beijing/1/1994 (Ck/BJ/1/94)-like viruses circulating in poultry in eastern China before 1998 have been gradually replaced by A/Chicken/Shanghai/F/1998 (Ck/SH/F/98)-like viruses, which have a genotype different from that of viruses isolated in southern China. The similarity of the internal genes of these H9N2 viruses to those of the H5N1 influenza viruses isolated from 2001 onwards suggests that the Ck/SH/F/98-like virus may have been the donor of internal genes of human and poultry H5N1 influenza viruses circulating in Eurasia. Experimental studies showed that some of these H9N2 viruses could be efficiently transmitted by the respiratory tract in chicken flocks. Our study provides new insight into the genesis and evolution of H9N2 influenza viruses and supports the notion that some of these viruses may have been the donors of internal genes found in H5N1 viruses.

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* Corresponding author. Mailing address: Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, 48 East Wenhui Road, Yangzhou, Jiangsu 225009, China. Phone: (86) 0514-87991416. Fax: (86) 0514-87972591. E-mail: xfliu{at}yzu.edu.cn

Published ahead of print on 24 June 2009.

Supplemental material for this article may be found at http://jvi.asm.org/.

Present address: Department of Microbiology and Immunology and Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107.

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Journal of Virology, September 2009, p. 8428-8438, Vol. 83, No. 17
0022-538X/09/$08.00+0     doi:10.1128/JVI.00659-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.