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Journal of Virology, August 2009, p. 8289-8292, Vol. 83, No. 16
0022-538X/09/$08.00+0     doi:10.1128/JVI.00709-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Evidence that Ecotropic Murine Leukemia Virus Contamination in TZM-bl Cells Does Not Affect the Outcome of Neutralizing Antibody Assays with Human Immunodeficiency Virus Type 1 {triangledown}

Emily J. Platt,1 Miroslawa Bilska,2 Susan L. Kozak,1 David Kabat,1 and David C. Montefiori2*

Department of Biochemistry and Molecular Biology, Oregon Health Sciences University, Portland, Oregon 97201,1 Department of Surgery, Duke University Medical Center, Durham, North Carolina 277102

Received 6 April 2009/ Accepted 19 May 2009

The TZM-bl cell line that is commonly used to assess neutralizing antibodies against human immunodeficiency virus type 1 (HIV-1) was recently reported to be contaminated with an ecotropic murine leukemia virus (MLV) (Y. Takeuchi, M. O. McClure, and M. Pizzato, J. Virol. 82:12585-12588, 2008), raising questions about the validity of results obtained with this cell line. Here we confirm this observation and show that HIV-1 neutralization assays performed with a variety of serologic reagents in a similar cell line that does not harbor MLV yield results that are equivalent to those obtained in TZM-bl cells. We conclude that MLV contamination has no measurable effect on HIV-1 neutralization when TZM-bl cells are used as targets for infection.

* Corresponding author. Mailing address: Department of Surgery, Laboratory for AIDS Vaccine Research and Development, P.O. Box 2926, Duke University Medical Center, Durham, NC 27710. Phone: (919) 684-5278. Fax: (919) 684-4288. E-mail: monte{at}duke.edu

{triangledown} Published ahead of print on 27 May 2009.

Journal of Virology, August 2009, p. 8289-8292, Vol. 83, No. 16
0022-538X/09/$08.00+0     doi:10.1128/JVI.00709-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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