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Journal of Virology, August 2009, p. 8270-8275, Vol. 83, No. 16
0022-538X/09/$08.00+0     doi:10.1128/JVI.00670-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Truncation of TRIM5 in the Feliformia Explains the Absence of Retroviral Restriction in Cells of the Domestic Cat{triangledown}

William A. McEwan,1* Torsten Schaller,2 Laura M. Ylinen,2 Margaret J. Hosie,1 Greg J. Towers,2 and Brian J. Willett1

Retrovirus Research Laboratory, Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, United Kingdom,1 MRC Centre for Medical Molecular Virology, Department of Infection, Royal Free and University College Medical School, University College London, 46 Cleveland Street, London, United Kingdom2

Received 1 April 2009/ Accepted 26 May 2009

TRIM5{alpha} mediates a potent retroviral restriction phenotype in diverse mammalian species. Here, we identify a TRIM5 transcript in cat cells with a truncated B30.2 capsid binding domain and ablated restrictive function which, remarkably, is conserved across the Feliformia. Cat TRIM5 displayed no restriction activity, but ectopic expression conferred a dominant negative effect against human TRIM5{alpha}. Our findings explain the absence of retroviral restriction in cat cells and suggest that disruption of the TRIM5 locus has arisen independently at least twice in the Carnivora, with implications concerning the evolution of the host and pathogen in this taxon.

* Corresponding author. Mailing address: Retrovirus Research Laboratory, Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Glasgow G61 1QH, United Kingdom. Phone: 44 141 330 5610. Fax: 44 141 942 7215. E-mail: w.mcewan.1{at}research.gla.ac.uk

{triangledown} Published ahead of print on 3 June 2009.

Journal of Virology, August 2009, p. 8270-8275, Vol. 83, No. 16
0022-538X/09/$08.00+0     doi:10.1128/JVI.00670-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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