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Journal of Virology, August 2009, p. 8247-8253, Vol. 83, No. 16
0022-538X/09/$08.00+0     doi:10.1128/JVI.00897-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Ultradeep Pyrosequencing Detects Complex Patterns of CD8+ T-Lymphocyte Escape in Simian Immunodeficiency Virus-Infected Macaques{triangledown} ,{dagger}

Benjamin N. Bimber,1,{ddagger} Benjamin J. Burwitz,2,{ddagger} Shelby O'Connor,2,{ddagger} Ann Detmer,1 Emma Gostick,3 Simon M. Lank,1 David A. Price,3 Austin Hughes,4 and David O'Connor1,2*

Wisconsin National Primate Research Center, University of Wisconsin—Madison, Madison, Wisconsin 53706,1 Department of Pathology and Laboratory Medicine, University of Wisconsin—Madison, Madison, Wisconsin 53706,2 Department of Medical Biochemistry and Immunology, Cardiff University School of Medicine, Heath Park, Cardiff, United Kingdom,3 Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 292084

Received 5 May 2009/ Accepted 1 June 2009

Human and simian immunodeficiency viruses (HIV/SIV) exhibit enormous sequence heterogeneity within each infected host. Here, we use ultradeep pyrosequencing to create a comprehensive picture of CD8+ T-lymphocyte (CD8-TL) escape in SIV-infected macaques, revealing a previously undetected complex pattern of viral variants. This increased sensitivity enabled the detection of acute CD8-TL escape as early as 17 days postinfection, representing the earliest published example of CD8-TL escape in intrarectally infected macaques. These data demonstrate that pyrosequencing can be used to study the evolution of CD8-TL escape during immunodeficiency virus infection with an unprecedented degree of sensitivity.

* Corresponding author. Mailing address: University of Wisconsin—Madison, 555 Science Drive, Madison, Wisconsin 53711. Phone: (608) 890-0845. Fax: (608) 265-8084. E-mail: doconnor{at}primate.wisc.edu

{triangledown} Published ahead of print on 10 June 2009.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

{ddagger} These authors contributed equally to the manuscript.

Journal of Virology, August 2009, p. 8247-8253, Vol. 83, No. 16
0022-538X/09/$08.00+0     doi:10.1128/JVI.00897-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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