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Journal of Virology, August 2009, p. 7796-7804, Vol. 83, No. 16
0022-538X/09/$08.00+0     doi:10.1128/JVI.00745-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Virion-Incorporated Glycoprotein B Mediates Transneuronal Spread of Pseudorabies Virus {triangledown} ,{dagger}

Dusica Curanovic and Lynn W. Enquist*

Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544

Received 10 April 2009/ Accepted 22 May 2009

Transneuronal spread of pseudorabies virus (PRV) is a multistep process that requires several virally encoded proteins. Previous studies have shown that PRV glycoprotein B (gB), a component of the viral fusion machinery, is required for the transmission of infection to postsynaptic, second-order neurons. We sought to identify the gB-mediated step in viral transmission. We determined that gB is not required for the sorting of virions into axons of infected neurons, anterograde transport, or the release of virions from the axon. trans or cis expression of gB on the cell surface was not sufficient for transneuronal spread of the virus; instead, efficient incorporation of gB into virions was required. Additionally, neuron-to-cell spread of PRV most likely does not proceed through syncytial connections. We conclude that, upon gB-independent release of virions at the site of neuron-cell contacts, the virion-incorporated gB/gH/gL fusion complex mediates entry into the axonally contacted cell by fusion of the closely apposed membranes.

* Corresponding author. Mailing address: 314 Schultz Laboratory, Department of Molecular Biology, Princeton University, Princeton, NJ 08544. Phone: (609) 258-2415. Fax: (609) 258-1035. E-mail: lenquist{at}princeton.edu

{triangledown} Published ahead of print on 3 June 2009.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, August 2009, p. 7796-7804, Vol. 83, No. 16
0022-538X/09/$08.00+0     doi:10.1128/JVI.00745-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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