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Journal of Virology, August 2009, p. 7789-7793, Vol. 83, No. 15
0022-538X/09/$08.00+0     doi:10.1128/JVI.00473-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Modulation of Human Immunodeficiency Virus Type 1 Protease Autoprocessing by Charge Properties of Surface Residue 69{triangledown}

Liangqun Huang,1 Jane M. Sayer,2 Marie Swinford,1 John M. Louis,2* and Chaoping Chen1*

Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523-1870,1 Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 208922

Received 6 March 2009/ Accepted 13 May 2009

Mature, fully active human immunodeficiency virus protease (PR) is liberated from the Gag-Pol precursor via regulated autoprocessing. A chimeric protease precursor, glutathione S-transferase-transframe region (TFR)-PR-FLAG, also undergoes N-terminal autocatalytic maturation when it is expressed in Escherichia coli. Mutation of the surface residue H69 to glutamic acid, but not to several neutral or basic amino acids, impedes protease autoprocessing in bacteria and mammalian cells. Only a fraction of mature PR with an H69E mutation (PRH69E) folds into active enzymes, and it does so with an apparent Kd (dissociation constant) significantly higher than that of the wild-type protease, corroborating the marked retardation of the in vitro N-terminal autocatalytic processing of TFR-PRH69E and suggesting a folding defect in the precursor.

* Corresponding author. Mailing address for Chaoping Chen: Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523-1870. Phone: (970) 491-0726. Fax: (970) 491-0454. E-mail: chaoping{at}colostate.edu. Mailing address for John M. Louis: Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892. Phone: (301) 594-3122. Fax: (301) 480-4001. E-mail: johnl{at}niddk.nih.gov

{triangledown} Published ahead of print on 20 May 2009.

Journal of Virology, August 2009, p. 7789-7793, Vol. 83, No. 15
0022-538X/09/$08.00+0     doi:10.1128/JVI.00473-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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