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Journal of Virology, July 2009, p. 7361-7364, Vol. 83, No. 14
0022-538X/09/$08.00+0     doi:10.1128/JVI.02662-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification of Varicella-Zoster Virus-Specific CD8 T Cells in Patients after T-Cell-Depleted Allogeneic Stem Cell Transplantation{triangledown}

Pim L. J. van der Heiden,1* Renate de Boer,1 Dirk M. van der Steen,1 Michel G. D. Kester,1 Menno W. A. G. van der Hoorn,1 Wilmy M. E. Haarman,1 Helen E. Barnby-Porritt,2 Jeremy W. Fry,2 C. E. Napper,2 Erik W. A. Marijt,1 Roel Willemze,1 J. H. Frederik Falkenburg,1 and Mirjam H. M. Heemskerk1

Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands,1 ProImmune Limited, Oxford, United Kingdom2

Received 27 December 2008/ Accepted 14 April 2009

To study the role of CD8 T cells in the control of varicella-zoster virus (VZV) reactivation, we developed multimeric major histocompatibility complexes to identify VZV-specific CD8 T cells. Potential HLA-A2 binding peptides from the putative immediate-early 62 protein (IE62) of VZV were tested for binding, and peptides with sufficient binding capacity were used to generate pentamers. Patients with VZV reactivation following stem cell transplantation were screened with these pentamers, leading to the identification of the first validated class I-restricted epitope of VZV. In 42% of HLA-A2 patients following VZV reactivation, these IE62-ALW-A2 T cells could be detected ex vivo.

* Corresponding author. Mailing address: Leiden University Medical Center, Department of Hematology, C2-R Albinusdreef 2, 2333 ZA Leiden, The Netherlands. Phone: 31 71 526 2267. Fax: 31 71 526 6755. E-mail: P.L.J.van_der_Heiden{at}lumc.nl

{triangledown} Published ahead of print on 22 April 2009.

Journal of Virology, July 2009, p. 7361-7364, Vol. 83, No. 14
0022-538X/09/$08.00+0     doi:10.1128/JVI.02662-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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