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Journal of Virology, July 2009, p. 6978-6986, Vol. 83, No. 14
0022-538X/09/$08.00+0     doi:10.1128/JVI.00598-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Comparison of the Pseudorabies Virus Us9 Protein with Homologs from Other Veterinary and Human Alphaherpesviruses {triangledown}

M. G. Lyman, C. D. Kemp,{dagger} M. P. Taylor, and L. W. Enquist*

Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544

Received 23 March 2009/ Accepted 24 April 2009

Pseudorabies virus (PRV) Us9 is a small, tail-anchored (TA) membrane protein that is essential for axonal sorting of viral structural proteins and is highly conserved among other members of the alphaherpesvirus subfamily. We cloned the Us9 homologs from two human pathogens, varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1), as well as two veterinary pathogens, equine herpesvirus type 1 (EHV-1) and bovine herpesvirus type 1 (BHV-1), and fused them to enhanced green fluorescent protein to examine their subcellular localization and membrane topology. Akin to PRV Us9, all of the Us9 homologs localized to the trans-Golgi network and had a type II membrane topology (typical of TA proteins). Furthermore, we examined whether any of the Us9 homologs could compensate for the loss of PRV Us9 in anterograde, neuron-to-cell spread of infection in a compartmented chamber system. EHV-1 and BHV-1 Us9 were able to fully compensate for the loss of PRV Us9, whereas VZV and HSV-1 Us9 proteins were unable to functionally replace PRV Us9 when they were expressed in a PRV background.

* Corresponding author. Mailing address: 314 Schultz Laboratory, Department of Molecular Biology, Princeton, NJ 08544. Phone: (609) 258-2415. Fax: (609) 258-1035. E-mail: lenquist{at}princeton.edu

{triangledown} Published ahead of print on 6 May 2009.

{dagger} Present address: Department of Surgery, The Johns Hopkins Hospital, Baltimore, MD 21287.

Journal of Virology, July 2009, p. 6978-6986, Vol. 83, No. 14
0022-538X/09/$08.00+0     doi:10.1128/JVI.00598-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • McGraw, H. M., Awasthi, S., Wojcechowskyj, J. A., Friedman, H. M. (2009). Anterograde Spread of Herpes Simplex Virus Type 1 Requires Glycoprotein E and Glycoprotein I but Not Us9. J. Virol. 83: 8315-8326 [Abstract] [Full Text]  


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