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Journal of Virology, July 2009, p. 6917-6921, Vol. 83, No. 13
0022-538X/09/$08.00+0     doi:10.1128/JVI.00081-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Enumeration of an Extremely High Particle-to-PFU Ratio for Varicella-Zoster Virus{triangledown} ,{dagger}

John E. Carpenter, Ernesto P. Henderson, and Charles Grose*

Central Microscopy Research Facility, University of Iowa, Iowa City, Iowa 52242

Received 13 January 2009/ Accepted 9 April 2009

Varicella-zoster virus (VZV) is renowned for its low titers. Yet investigations to explore the low infectivity are hampered by the fact that the VZV particle-to-PFU ratio has never been determined with precision. Herein, we accomplish that task by applying newer imaging technology. More than 300 images were taken of VZV-infected cells on 4 different samples at high magnification. We enumerated the total number of viral particles within 25 cm2 of the infected monolayer at 415 million. Based on these numbers, the VZV particle:PFU ratio was approximately 40,000:1 for a cell-free inoculum.

* Corresponding author. Mailing address: University of Iowa Hospital, 200 Hawkins Drive, Iowa City, IA 52242. Phone: (319) 356-2270. Fax: (319) 356-4855. E-mail: charles-grose{at}uiowa.edu

{triangledown} Published ahead of print on 15 April 2009.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, July 2009, p. 6917-6921, Vol. 83, No. 13
0022-538X/09/$08.00+0     doi:10.1128/JVI.00081-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Walters, M. S., Erazo, A., Kinchington, P. R., Silverstein, S. (2009). Histone Deacetylases 1 and 2 Are Phosphorylated at Novel Sites during Varicella-Zoster Virus Infection. J. Virol. 83: 11502-11513 [Abstract] [Full Text]  


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