This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Pasdeloup, D.
Right arrow Articles by Rixon, F. J.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pasdeloup, D.
Right arrow Articles by Rixon, F. J.

 Previous Article  |  Next Article 

Journal of Virology, July 2009, p. 6610-6623, Vol. 83, No. 13
0022-538X/09/$08.00+0     doi:10.1128/JVI.02655-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Herpesvirus Capsid Association with the Nuclear Pore Complex and Viral DNA Release Involve the Nucleoporin CAN/Nup214 and the Capsid Protein pUL25{triangledown}

David Pasdeloup,1,2* Danielle Blondel,2 Anabela L. Isidro,2,3 and Frazer J. Rixon1

MRC Virology Unit, Church Street, Glasgow G11 5JR, United Kingdom,1 Laboratoire de Virologie Moléculaire et Structurale, CNRS, Avenue de la Terrasse, 91198 Gif-Sur-Yvette, France,2 Instituto de Tecnologia Química e Biológica, Oeiras, Portugal3

Received 24 December 2008/ Accepted 14 April 2009

After penetrating the host cell, the herpesvirus capsid is transported to the nucleus along the microtubule network and docks to the nuclear pore complex before releasing the viral DNA into the nucleus. The viral and cellular interactions involved in the docking process are poorly characterized. However, the minor capsid protein pUL25 has recently been reported to be involved in viral DNA uncoating. Here we show that herpes simplex virus type 1 (HSV-1) capsids interact with the nucleoporin CAN/Nup214 in infected cells and that RNA silencing of CAN/Nup214 delays the onset of viral DNA replication in the nucleus. We also show that pUL25 interacts with CAN/Nup214 and another nucleoporin, hCG1, and binds to the pUL36 and pUL6 proteins, two other components of the herpesvirus particle that are known to be important for the initiation of infection and viral DNA release. These results identify CAN/Nup214 as being a nuclear receptor for the herpesvirus capsid and pUL25 as being an interface between incoming capsids and the nuclear pore complex and as being a triggering element for viral DNA release into the nucleus.

* Corresponding author. Mailing address: MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, United Kingdom. Phone: 44-141-330-4025. Fax: 44-141-337-2236. E-mail: d.pasdeloup{at}mrcvu.gla.ac.uk

{triangledown} Published ahead of print on 22 April 2009.

Journal of Virology, July 2009, p. 6610-6623, Vol. 83, No. 13
0022-538X/09/$08.00+0     doi:10.1128/JVI.02655-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»