This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Google Scholar
Right arrow Articles by Johnson, L. A.
Right arrow Articles by Sandri-Goldin, R. M.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johnson, L. A.
Right arrow Articles by Sandri-Goldin, R. M.

 Previous Article  |  Next Article 

Journal of Virology, July 2009, p. 6335-6346, Vol. 83, No. 13
0022-538X/09/$08.00+0     doi:10.1128/JVI.00375-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

The Cellular RNA Export Receptor TAP/NXF1 Is Required for ICP27-Mediated Export of Herpes Simplex Virus 1 RNA, but the TREX Complex Adaptor Protein Aly/REF Appears To Be Dispensable{triangledown}

Lisa A. Johnson,{dagger} Ling Li,{dagger},{ddagger} and Rozanne M. Sandri-Goldin*

Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, California 92697-4025

Received 19 February 2009/ Accepted 7 April 2009

Herpes simplex virus 1 (HSV-1) protein ICP27 has been shown to shuttle between the nucleus and cytoplasm and to bind viral RNA during infection. ICP27 was found to interact with the cellular RNA export adaptor protein Aly/REF, which is part of the TREX complex, and to relocalize Aly/REF to viral replication sites. ICP27 is exported to the cytoplasm through the export receptor TAP/NXF1, and ICP27 must be able to interact with TAP/NXF1 for efficient export of HSV-1 early and late transcripts. We examined the dynamics of ICP27 movement and its localization with respect to Aly/REF and TAP/NXF1 in living cells during viral infection. Recombinant viruses with a yellow fluorescent protein (YFP) tag on the N or C terminus of ICP27 were constructed. While the N-terminally tagged ICP27 virus behaved like wild-type HSV-1, the C-terminally tagged virus was defective in viral replication and gene expression, and ICP27 was confined to the nucleus, suggesting that the C-terminal YFP tag interfered with ICP27's C-terminal interactions, including the interaction with TAP/NXF1. To assess the role of Aly/REF and TAP/NXF1 in viral RNA export, these factors were knocked down using small interfering RNA. Knockdown of Aly/REF had little effect on the export of ICP27 or poly(A)+ RNA during infection. In contrast, a decrease in TAP/NXF1 levels severely impaired export of ICP27 and poly(A)+ RNA. We conclude that TAP/NXF1 is essential for ICP27-mediated export of RNA during HSV-1 infection, whereas Aly/REF may be dispensable.

* Corresponding author. Mailing address: Department of Microbiology & Molecular Genetics, School of Medicine, Medical Sciences I, Rm. B240, University of California at Irvine, Irvine, CA 92697-4025. Phone: (949) 824-7570. Fax: (949) 824-9054. E-mail: rmsandri{at}uci.edu

{triangledown} Published ahead of print on 15 April 2009.

{dagger} L.A.J. and L.L. contributed equally to this work.

{ddagger} Present address: Verdezyne, Inc., Carlsbad, CA.

Journal of Virology, July 2009, p. 6335-6346, Vol. 83, No. 13
0022-538X/09/$08.00+0     doi:10.1128/JVI.00375-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Juillard, F., Hiriart, E., Sergeant, N., Vingtdeux-Didier, V., Drobecq, H., Sergeant, A., Manet, E., Gruffat, H. (2009). Epstein-Barr Virus Protein EB2 Contains an N-Terminal Transferable Nuclear Export Signal That Promotes Nucleocytoplasmic Export by Directly Binding TAP/NXF1. J. Virol. 83: 12759-12768 [Abstract] [Full Text]  
  • Souki, S. K., Sandri-Goldin, R. M. (2009). Arginine Methylation of the ICP27 RGG Box Regulates the Functional Interactions of ICP27 with SRPK1 and Aly/REF during Herpes Simplex Virus 1 Infection. J. Virol. 83: 8970-8975 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»